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Facutly and Post Doc List > Dr. Donald Burke > Dr. Donald Burke Research > DARS

DARS: Database of Aptamer and Ribozyme Sequences Introduction:

During the course of in vitro selections, we generate a lot of sequences. Some of these we understand fairly well, and most we do not. Certainly most are not published in their entirety in the primary research literature.

The raw sequence data available with two purposes in mind:

First, by this mechanism you can have access to more sequence information than typically makes its way into the primary research literature, perhaps facilitating your efforts to synthesize some of these molecules for yourself.

Second, the community is invited to some hidden lesson these sequences may hold, either by using a different strategy than what we have tried, or by being plain smarter. Either way, Bonne Chance!

The two requests that I make are 1) that you let me know (burkedh@missouri.edu) when you find something hidden in the sequence data (the sooner the better, athough perhaps after you've confirmed your find and it seems to be on solid ground); and 2) that you acknowledge your data source when discussing your results (talks, meetings, publications, etc.).

This material is based upon work supported in part by the National Science Foundation under Grant No. 9896363, by the National Institute of Allergies and Infectious Diseases under Grant No. AI45344, by the Arnold and Mabel Beckman Young Investigators Award, and by Indiana University.

The following data sets are currently available:

Aptamers selected to bind FAD or GMP, evolved from mutated FAD or FMN aptamers Submitted for publication

Aptamers to ATP selected with boronated nucleotide analogs, published in Nuc. Ac. Res., 2002, 30:1401-1407.

Aptamers selected to bind FAD, published in Biochemistry, 2002, 41:2492-2499.

70Cm and 80Cm aptamers to chloramphenicol published in Chem & Biol, 1997.

80SAM Aptamers to S-adenosyl methionine

Aptamers to coenzymeA (70A, 80A, 70A100, & 80A100 pools) published in Biochemistry, 1998.

Aptamers to coenzymeA, selected on disulfide-linked CoA affinity matrix (70SA, 80SA, 70PSA, & 80PSA pools) Directed & Applied Evolution, 2003 (in press).

Bifunctional aptamers selected to bind both CoA and Cm or both adenosine and Cm

Aptamers to the protein HIV reverse transcriptase